Composite

Part:BBa_K3840312

Designed by: Nefeli Zikou   Group: iGEM21_Greece_United   (2021-10-01)


Lamp2b Signal Peptide – CAP – GFP – Lamp2b – mir140

This composite part is a fusion of 4 peptides producing a Lamp2b protein fused with a GFP and a Chondrocyte Affinity Peptide. This part also has the information for the production of microRNA-140 This leads to the production of exosomes with the modified Lamp2b in their membrane, and the microRNA-140 inside the exosomes. The modified Lamp-2b due to the Chondrocyte Affinity Peptide targets specifically Chondrocytes releasing their cargo, while micro-140 inhibits the expression of certain proteins which promote the progression of Osteoarthritis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 889
    Illegal XhoI site found at 571
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 82
    Illegal NgoMIV site found at 1468
    Illegal NgoMIV site found at 1957
    Illegal NgoMIV site found at 2023
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None